Cholesterol Metabolism and Efflux in Human THP-1 Macrophages
نویسندگان
چکیده
منابع مشابه
Cholesterol metabolism and efflux in human THP-1 macrophages.
This study has investigated in detail factors regulating accumulation, esterification, and mobilization of cholesterol in human THP-1 macrophages. Human THP-1 monocytes were differentiated into macrophages and then cholesterol enriched by exposure to acetylated LDL (AcLDL), together with [3H]free cholesterol (FC). Although THP-1 macrophages accumulated FC and esterified cholesterol (EC), assess...
متن کاملCharacterization of fluorescent NBD‑cholesterol efflux in THP‑1‑derived macrophages.
Macrophage cholesterol efflux is important in maintaining cellular lipid homeostasis and preventing the formation of lipid‑laden foam cells. Although radioactive [3H]‑cholesterol is widely used as a tracer in cholesterol efflux assays, the lengthy and labor‑intensive assay procedure, and the radioactivity disposal procedure limit the use of this assay for high‑throughput screening. In the prese...
متن کاملPiperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages
SCOPE Increased macrophage cholesterol efflux (ChE) is considered to have anti-atherosclerotic effect counteracting cardiovascular disease. The principle pungent ingredient of the fruits of Piper nigrum, piperine, is identified in this study as a ChE inducer in THP-1-derived macrophages, and mechanisms underlying this effect are explored. METHODS AND RESULTS Without affecting cell viability, ...
متن کاملEndothelial lipase promotes apolipoprotein AI-mediated cholesterol efflux in THP-1 macrophages.
OBJECTIVE Endothelial lipase (EL) is expressed by macrophages within atherosclerotic lesions. We investigated the influence of EL expression on cholesterol efflux in macrophages. METHODS AND RESULTS The present study used lentivirus to introduce either EL shRNA for loss-of-function studies or EL cDNA for gain-of-function studies to investigate the role of EL in apoAI-mediated cholesterol effl...
متن کاملthe effect of unsaturated fatty acids on molecular markers of cholesterol homeostasis in thp-1 macrophages
discussions in comparison to the control of wy14643, sandoz58-035, c9 and t11-cla, epa increased the pparα mrna levels (p = 0.024, p = 0.041, p = 0.043, and p = 0.004, respectively), even though, la had no significant effect on the pparα mrna expression (p = 0.489). materials and methods this experimental study investigated the effects of c9, t11-conjugated linoleic acid (c9, t11-cla). alpha li...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology
سال: 1998
ISSN: 1079-5642,1524-4636
DOI: 10.1161/01.atv.18.10.1589